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Northern-blot analysis of the annexin gene expression in cotton. Radioactive probe was generated from the full length of cotton annexin cDNA. Note that the expression of annexin gradually decreases as cotton fibers develop into the secondary stage. Annexin is highly expressed in flower and root tissues, but not in leaves (Leaf). Total RNA was quantitated by spectrophotometry, and equal loading (20 μg/lane) was confirmed by ethidium bromide staining.
