Table I.
Influence of recombinant cotton annexin on β-glucan synthase activity
| Enzyme Fraction | 100% of β-Glucan Synthase Activity before Adding Annexin | β-Glucan Synthase Activity after Adding Annexin |
|---|---|---|
| units/mg | % | |
| PME | 20.1 | 100 ± 6.2 |
| SE1 | 3.1 | 100 ± 5.5 |
| SE2 | 12.5 | 100 ± 4.2 |
| P | 15.2 | 100 ± 5.1 |
In each experiment, three separate reactions were carried out with individual enzyme fractions by using 1, 5, and 10 μg of purified recombinant annexin. The same experiment was repeated three times to obtain sd values. 1 unit = 1 nmol Glc incorporated min−1. SE1, The first solubilized fraction with 0.05% digitonin; SE2, the second solubilized fraction with 1% digitonin; P, the pellet fraction that remained after solubilization.