Table II.
Recombinant annexin and ATPase/GTPase activity
| Samples | ATPase Activity | GTPase Activity |
|---|---|---|
| nmol Pi mg−1 h−1 | ||
| Purified whole annexin | ||
| Ann1F4R | 42.5 ± 3.3 | 400.1 ± 5.2 |
| Heat-treated whole annexin | ||
| Ann1F4R | 5.1 ± 2.1 | 8.3 ± 2.5 |
| N-terminal deletion mutants | ||
| Ann2F4R | 2.1 ± 1.1 | 1.5 ± 2.2 |
| Ann3F4R | 3.2 ± 1.3 | 2.1 ± 0.7 |
| C-terminal deletion mutants | ||
| Ann1F3R | 1.3 ± 1.5 | 2 ± 1.5 |
| Ann1F2R | 2.5 ± 1.6 | 1.7 ± 0.5 |
ATPase and GTPase assays were performed at pH 6.5, with 10 μg of purified recombinant annexin proteins and 3 mm MgCl2 in the presence of 3 mm ATP or GTP. At least three replicates were carried out for each assay to obtain sd values.