Figure 4. Schematic sequence showing the use of SeroFluke strips with whole blood.

The sample (10 µl) was diluted 1/20 with SeroFluke buffer in a polystyrene microtitre plate well and allowed to haemolyze for 2 min (1, 2). The LFIA strip was then placed in the well, and allowed to react for 10 min (3). The strip was then cut at the beginning of the nitrocellulose membrane (4) and transferred to a new well containing 200 µl of fresh SeroFluke buffer (5). The results were read after washing the strip for another 10 min.