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Seventeen, 82, and 28 sequences from 2008 (pink), 2009 (blue) and 2010 (teal), respectively, were aligned using Muscle and clustered using phyML. Six isolates from Venezuela (“Ven”), Martinique (“Martinique”), Thailand (“Thai”) and Puerto Rico (“PR”) from the Asian-American genotype are also shown to root the tree and demonstrate similarities among the Nicaraguan isolates.
