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. 2011 Nov 8;6(11):e27074. doi: 10.1371/journal.pone.0027074

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Han et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Cells were exposed to medium with glucose concentrations varying from 5.5 to 50 mM for 12, 24, or 48 h. MTT assay was analyzed for proliferation rates (5.5 mM as a control). (A, B) shows the proliferation rates corresponding to different glucose concentrations in BxPC-3 and Panc-1 cells. (C, D) shows the proliferation rates corresponding to different glucose concentrations in BxPC-3 and Panc-1 cells treated with EGF-neutralizing antibody (* p<0.05 compared with group in 5.5 mM glucose; ^# p<0.05 compared with group in 25 mM glucose; n = 8 per group). (E, F) shows the comparison of proliferation rates before and after treating with EGF-neutralizing antibody with the different glucose concentrations in BxPC-3 and Panc-1 cells at the time point of 24 h (* p<0.05 compared with group of without EGF neutralizing antibody).