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. 2011 Nov 8;6(11):e27219. doi: 10.1371/journal.pone.0027219

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Lan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The high-field shifted region of 1D 1H NMR spectra of the native cyt c (down) and its P71H variant (upper) in oxidized state. (B) The down-field shifted region of 1D 1H NMR spectra of the native cyt c (down) and its P71H variant (upper) in oxidized state. The peaks in 1D 1H-NMR spectrum were assigned as (1a) His18 Hε1, (2a) His71 Hε1, (3a) His71 Hδ2, (4a) heme 8-CH3, (5a) His18 Hδ2, (6a) heme 3-CH3, (7a) His71 Hδ1, (8a) His18 Hδ1 and (9a) heme 5-CH3, respectively. The peaks in 1D 1H-NMR spectrum of native cyt c were assigned as (1b) His18 Hε1, (2b) Met80 ε-CH3, (3b) Met80 Hγ, (4b) heme 8-CH3, (5b) heme 3-CH3, (6b) His18 Hδ2, (7b) heme 7-Hα2, (8b) His18 Hβ2, (9b) heme 7-Hα1 and (10b) His18 Hδ1, respectively.