Table 3. The assignment of heme protons of cyt c P71H mutant in the oxidized state.
atoms | oxidized native cyt c (ppm) | oxidized P71H mutant (ppm) |
1-CH3 | 7.57 | 11.27 |
2-CH | −0.95 | −0.78 |
2-CH3 | −2.27 | 0.14 |
meso- Hα | 2.97 | 3.59 |
3-CH3 | 31.92 | 18.48 |
4-CH | 2.08 | 1.61 |
4-CH3 | 2.30 | 1.53 |
meso- Hβ | −0.42 | −0.41 |
5-CH3 | 10.75 | 13.62 |
6- Hα 1 | −1.55 | −3.07 |
6- Hα 2 | 2.06 | 0.62 |
6- Hβ 1 | −0.51 | 1.51 |
6- Hβ 2 | 0.97 | 2.26 |
meso- Hγ | 7.57 | 7.54 |
7- Hα 1 | 13.08 | 10.07 |
7- Hα 2 | 15.74 | 5.55 |
7- Hβ 1 | −0.20 | 2.52 |
7- Hβ 2 | 1.42 | 1.31 |
8-CH3 | 35.19 | 29.27 |
meso- Hδ | 2.03 | −2.74 |