Table 5. Kinetic parameters of VEGF and anti-VEGF.

	Measured parameters		Tissue parameters
VEGF binding to VEGFR-1	Value	Unit	Value (Tissue)	Value (Blood)	Unit
kon	3.00×107	M−1s−1	2.65×10−1	6.15×10−2	(pmol/cm3 tissue)−1s−1
koff	1.00×10−3	s−1
Kd	33	pM	3.73×10−3	1.61×10−2	pmol/cm3 tissue
VEGF binding to VEGFR-2
kon	1.00×107	M−1s−1	8.84×10−2	2.05×10−2	(pmol/cm3 tissue)−1s−1
koff	1.00×10−3	s−1
Kd	1.00×102	pM	1.13×10−2	4.88×10−2	pmol/cm3 tissue
VEGF164 binding to NRP-1
kon	3.13×106	M−1s−1	2.77×10−2	6.41×10−3	(pmol/cm3 tissue)−1s−1
koff	1.00×10−3	s−1
Kd	3.12×102	pM	3.52×10−2	1.52×10−1	pmol/cm3 tissue
VEGF164 binding to GAGs
kon	4.20×105	M−1s−1	3.72×10−3	-	(pmol/cm3 tissue)−1s−1
koff	1.00×10−2	s−1
Kd	2.40×101	nM	2.71	-	pmol/cm3 tissue
Coupling of NRP-1 and VEGFR-2
kc V164R2,N1	3.10×1013	(mol/cm2)−1s−1	1.99×10−1	1.59×10−2	(pmol/cm3 tissue)−1s−1
koff V164R2,N1	1.00×10−3	s−1
kc V164N1,R2	1.00×1014	(mol/cm2)−1s−1	6.41×10−1	5.13×10−2	(pmol/cm3 tissue)−1s−1
koff V164N1,R2	1.00×10−3	s−1
Coupling of NRP-1 and VEGFR-1
kc R1,N1	1.00×1014	(mol/cm2)−1s−1	6.41×10−1	5.13×10−2	(pmol/cm3 tissue)−1s−1
kdissoc R1,N1	1.00×10−2	s−1
VEGFR internalization
kint, R	2.80×10−4	s−1
kint, C	2.80×10−4	s−1
VEGF binding to anti-VEGF *
kon	1.13×108	M−1s−1	1.00	2.32×10−1	(pmol/cm3 tissue)−1s−1
koff	4.23×10−5	s−1
Kd	0.37	pM	4.18×10−5	1.81×10−4	pmol/cm3 tissue

*Optimized parameter values.

Conversions:

Tissue: 1.13×10⁸ (pmol/cm³ tissue)/M and 1.56×10¹⁴ (pmol/cm³ tissue)/(mol/cm² EC).

Blood: 4.88×10⁸ (pmol/cm³ tissue)/M and 1.95×10¹⁵ (pmol/cm³ tissue)/(mol/cm² EC).