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. 2011 Nov 8;6(11):e27409. doi: 10.1371/journal.pone.0027409

Figure 1. Expression of viral MPs of the 30 K superfamily interferes with yeast cell growth.

Figure 1

Growth kinetics of WT yeast cells cultured in SD medium +/- Doxycycline (DOX) and transformed with empty vector (pCM) (A), a full-length MPpnrsv expressing plasmid (B) or a truncated MPpnrsvΔHR expressing plasmid (C). (D) Quantitation of cell growth in SD medium+/- DOX of yeast cultures transformed with vector alone or with expressing MPpnrsv and MPpnrsvΔH plasmids. (E) Quantitation of cell growth in SD medium+/- DOX, of WT yeasts transformed with either pCM (lane 1) or with plasmids expressing PNRSV, BMV, CMV, TMV and GLFV MPs (lanes 2, 3, 4, 5 and 6, respectively). Data in D and E, represent the average +/- standard error (s.e.) of at least three independent experiments, each one done in triplicate (p<0.001). (F) Immunodetection of MPpnrsv (lane 3) and MPpnrsvΔHR (lane 1) in protein extracts from yeast cells transformed with the corresponding expression vectors or pCM as negative control (lane 2). (G) The same as in (F), but in protein extracts from yeast cells transformed with pCM (lane 1) or plasmids expressing PNRSV, BMV, CMV, TMV or GLFV MPs (lanes 2, 3, 4, 5 and 6, respectively) Coomassie Blue (CB) stained gel used as loading control).