Figure 6. Role of ionic interactions in enolase binding of plasminogen.

Binding of plasminogen to immobilized enolase (10 µg/ml) was analyzed by ELISA. Plasminogen (25 µg/ml) was incubated in the presence of increasing concentrations of NaCl. Bound plasminogen was detected using a specific antiserum. BSA was used as a negative control. Data are presented as % control (physiological NaCl, 150 mM) from the means and standard errors from three experiments with six replicates per concentration of NaCl.