Figure 7. Enolase-bound plasminogen is converted into plasmin.

Enolase-coated wells of microtiter plates were incubated with plasminogen, urokinase (uPA), and/or a plasmin-specific chromogenic substrate. Proteolytic activity was measured by absorbance at 405 nM. Data represent the means and standard errors from three different experiments with six replicates per condition. ***, P<0.001 compared to the activation of plasminogen bound to the control protein, BSA, Student's t test assuming unequal variances.