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. 2011 Nov 8;6(11):e27503. doi: 10.1371/journal.pone.0027503

Figure 2. CFP10 : Rv3871 protein-protein interaction.

Figure 2

(A) Bacterial two-hybrid X-Gal plate showing co-transformants: CFP10pBTnn + ESAT6pTRGnn (positive control); CFP10pBTnn + Rv3871pTRGnn; CFP10-ESAT6pBTnn + Rv3871pTRGnn; and pBTnn + Rv3871pTRGnn (negative control). “+" denotes the fact that two or more proteins (or plasmids) are present together, either within a strain or in vitro. Two different colonies of each co-transformant were patched. (B) Quantitative analysis by liquid β-galactosidase assay. All interactions were found to be statistically significant (*, P<0.001; **, P<0.05).