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. 2011 Nov 8;6(11):e27503. doi: 10.1371/journal.pone.0027503

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Tharad et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) X-Gal Arabinose indicator plate showing triple co-transformants: CFP10pBTnn + Rv3871pTRGnn + ESAT6pMTSA; and CFP10pBTnn + Rv3871pTRGnn + pMTSA. Two separate colonies from each co-transformant plate were picked and patched. (B) Time course liquid β-galactosidase assay: Relative β-galactosidase activity of the triple co-transformants (▴) CFP10pBTnn + Rv3871pTRGnn + pMTSA; and (▪) CFP10pBTnn + Rv3871pTRGnn + ESAT6pMTSA is plotted against time-points of bacterial culture growth, with 0 time-point being the point of arabinose induction. The graph is the average of three independent assays and standard deviation is represented as error bars. (P<0.05 at all time points beyond 60 minutes). (C) Relative fold change of lacZ gene transcripts in Bacterial three-hybrid system: Approximately two-fold increase in lacZ gene transcription is observed in comparison to negative control when CFP10 and Rv3871 interact in the presence of ESAT6 in the three-hybrid system. (*, P<0.01).