Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 8;6(11):e27503. doi: 10.1371/journal.pone.0027503

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Tharad et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) X-Gal indicator plate with and without arabinose patched with ESAT6pBTnn + HCL1pTRGnn + pMTSA; and ESAT6pBTnn + HCL1pTRGnn + CFP10pMTSA. Blue colony turns white when CFP10 is allowed to express in the presence of 1% arabinose. (B) Time course liquid β-galactosidase assay: β-galactosidase activity of above triple co-transformants: (▪) ESAT6pBTnn + HCL1pTRGnn + pMTSA; and (•) ESAT6pBTnn + HCL1pTRGnn + CFP10pMTSA is plotted against time-points of bacterial culture growth with 0 time-point being the point of arabinose induction. Standard deviation of the activities obtained in three separate assays is shown by error bars. (P<0.05 at all time-points beyond 60 minutes) (C) Arabinose gradient liquid β-galactosidase assay: Relative β-galactosidase activity of triple co-transformants (•) ESAT6pBTnn + HCL1pTRGnn + CFP10pMTSA; and (▪) ESAT6pBTnn + HCL1pTRGnn + pMTSA is plotted against arabinose concentration. The graph is the average of three separate assays and standard deviation is represented as error bars. (P<0.05 at all arabinose concentrations beyond 0.01%).