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. 2011 Nov 8;6(11):e27522. doi: 10.1371/journal.pone.0027522

Figure 6. Species-specificity of vankyrin expression in various insect cell lines.

Figure 6

(A) Absolute qPCR analysis was performed to assay the four HdIV segments in calyx fluid (CF), cell cytoplasm (C) and nuclear (N) extracts from HdIV-infected insect cell lines. The copy number of each DNA segment (i.e. Hd31, Hd4, Hd27 and Hd4) was calculated using a standard curve with primers specific for Hd31-vank1, Hd4-vank1, Hd27-vank1 and Hd4-vank1, respectively. (B) Absolute qRT-PCR experiments were performed to analyze the expression levels of vankyrin genes in selected cell lines at 24 h p.i. Results were normalized to ng of DNA. No viral-specific amplification was detected for cDNA pools from non-infected cell lines (not shown). The detection level of the vankyrin mRNAs in C6/36 cells was close to those found in the non-infected conditions, indicating that there is no vankyrin gene expression in the dipteran cell line.