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. 2011 Nov 4;6(11):e27155. doi: 10.1371/journal.pone.0027155

Figure 3. Ac45 mediates fluid-phase endocytosis in osteoclasts and their precursor cells.

Figure 3

(A) Flow cytometric analysis of dextran-rhodamine B uptake by Ac45 siRNA treated BMM-derived osteoclasts and osteoclastic precursor cells. Cells transfected with siRNA oligos for 48hrs were treated with 50 µg/ml dextran-rhodamine B for 30mins followed by flow cytometric analysis. The percentage of cells with dextran-rhodamine B uptake was detected using 630/22nm bandpass with at least 10,000 events collected and analyzed to determine the proportion of cells containing dextran-rhodamine B and the geometric mean of the positive population. (B) Dextran-rhodamine B uptake by confocal microscopy. Osteoclasts treated with siRNA oligos were incubated with 50 µg/ml dextran-rhodamine B for 30mins prior to fixation for confocal analysis. Representative image of dextran-rhodamine B uptake by Ac45-silenced and control-treated osteoclasts from three independent experiments. Osteoclasts are indicated by the white arrowheads.