Figure 1. Species-specific regulation of AMP gene promoters in S2 and Sf9 cells.
S2 and Sf9 cells were transiently cotransfected with different reporter vectors using pRL-TK as an internal control. PG-K12 (peptidoglycan from E. coli K12 strain, 10 μg/ml final concentration) was used to stimulate cells for 48 h before luciferase activities were measured. Relative luciferase activity was normalized to the pGL-3B control group (arbitrarily set as 1). Bars represent the mean of three individual measurements ± S.E.M. Significance of difference between S2 and Sf9 cells in each group was determined by an unpaired t-test (* p<0.05; ** p<0.001).