TABLE 2.
Metabolite production in the strains using glycerol as a sole carbon source without coenzyme Q1 supplementation under aerobic conditions
| Strains | qmetabolite / qglycerola for:
|
Specific growth rate (h−1) | Biomass yield on glycerol (g/g) | ||
|---|---|---|---|---|---|
| Lactate | Acetate | Ethanol | |||
| GJT001 (pLOI295) | 0.00 ± 0.00 | 0.32 ± 0.18 | 0.52 ± 0.07 | 0.27 ± 0.03 | 1.85 ± 0.19 |
| GJT001(pCRADH2) | 0.04 ± 0.01 | 0.35 ± 0.01 | 0.20 ± 0.01 | 0.37 ± 0.05 | 0.91 ± 0.27 |
| YBS131(pLOI295) | 0.00 ± 0.00 | 0.29 ± 0.10 | 0.48 ± 0.04 | 0.33 ± 0.07 | 1.89 ± 0.17 |
| YBS131(pCRADH2) | 0.00 ± 0.00 | 0.42 ± 0.03 | 0.22 ± 0.01 | 0.42 ± 0.06 | 1.49 ± 0.39 |
| AMS001(pLOI295) | - b | - | - | - | - |
| AMS001(pCRADH2) | 0.64 ± 0.05 | 0.21 ± 0.12 | 0.17 ± 0.04 | 0.13 ± 0.01 | 0.16 ± 0.02 |
| AMS002(pLOI295) | 0.00 ± 0.00 | 0.17 ± 0.04 | 0.80 ± 0.06 | 0.07 ± 0.01 | 0.26 ± 0.01 |
| AMS002(pCRADH2) | 0.00 ± 0.00 | 0.53 ± 0.03 | 0.10 ± 0.02 | 0.05 ± 0.00 | 0.46 ± 0.03 |
a
qmetabolite denotes specific metabolite forming rates (mmol/ [g of dry weight]/h), and qglycerol denotes specific glycerol uptake rates (mmol/ [g of dry weight]/ h). These specific rates were calculated using the data during the mid-exponential phase of the cultures to qualify quasi-steady state assumption. The results represent the mean values ± standard deviations for measurements from at least three independent experiments.
b
The AMS001 (pLOI295) strain failed to grow without coenzyme Q1 supplementation under the experimental condition.