Fig. 2. Farnesol decreases biovolume, substratum coverage and mean thickness in biofilms in vitro.
The confocal images of the 24 h biofilms exposed to DMSO or farnesol were analyzed by PHLIP software, using MATLAB toolbox. The z stack images were analyzed for biovolume (µm3) (A) substratum coverage (%) (B) and mean thickness (µm) (C). Farnesol (□) decreased biovolume, substratum coverage and mean thickness significantly (p < 0.05), when compared to DMSO control (■) for all four S. epidermidis strains.