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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Biochemistry. 2011 Oct 14;50(45):9694–9707. doi: 10.1021/bi201276r

Figure 4.

Figure 4

Initial rates of Pi release (●) and oxaloacetate formation (▲) at varying concentrations of pyruvate (0.03-5.25 mM) were determined to evaluate the extent of coupling between the HCO3−-ATP cleavage and pyruvate carboxylation reactions in the presence (A) and absence (B) of saturating concentrations of acetyl-CoA. Solid lines indicate the least-square fits to eqn (1). Inset: Ratio of the rates of oxaloacetate formation and Pi release (○) as a function of pyruvate concentrations. Solid lines indicate the least-square fits to eqn (1).