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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Biochemistry. 2011 Oct 14;50(45):9694–9707. doi: 10.1021/bi201276r

Table 3.

Kinetic parameters determined from measuring the initial rates of pyruvate carboxylation with varying concentrations of acetyl-CoA at fixed concentrations of free Mg2+a.

Vmax
(μmol mg−1 min−1)		 Ka acetyl-CoA
(μM)		 nacetyl-CoA
0.5 mM Mg2+ 1.16 ± 0.07 38 ± 2 2.0 ± 0.3
1.0 mM Mg2+ 1.39 ± 0.06 26 ± 2 2.8 ± 0.5
1.5 mM Mg2+ 2.59 ± 0.08 25 ± 1 1.8 ± 0.2
3.0 mM Mg2+ 5.7 ± 0.1 10.0 ± 0.4 2.6 ± 0.2
a

Kinetic parameters were determined from data fits to eqn (4).