. Author manuscript; available in PMC: 2012 Nov 15.

Published in final edited form as: Biochemistry. 2011 Oct 14;50(45):9694–9707. doi: 10.1021/bi201276r

Table 4.

Effect of acetyl-CoA on the activities of wild-type catalyzed reactions^a.

	(+) acetyl-CoA	(−) acetyl-CoA
	k_cat(min⁻¹)^b	k_cat(min⁻¹)	% rate^c
Pyruvate carboxylation	700 ± 20	65.8 ± 0.4	9
Full reverse reaction	0.835 ± 0.005	0.152 ± 0.03	18
HCO₃⁻-dependent ATPase	2.58 ± 0.09	0.547 ± 0.002	22
Oxamate-induced decarboxylation of oxaloacetate	9.03 ± 0.04	6.00 ± 0.05	67
ADP phosphorylation	2.4 ± 0.1	2.43 ± 0.01	100

For detailed reaction conditions see methods section.

Specific activities were determined for each reaction, with saturating concentrations of all substrates, in triplicate. Errors reported are the standard deviations from those determinations.

Compared to the specific activity in the presence of acetyl-CoA.