. Author manuscript; available in PMC: 2012 Nov 15.

Published in final edited form as: Biochemistry. 2011 Oct 14;50(45):9694–9707. doi: 10.1021/bi201276r

Table 6.

Effect of free biotin on the kinetic parameters for the HCO₃⁻-dependent ATPase reaction catalyzed by the wild-type and mutant forms of RePC ^e.

					(+) 10 mM free biotin
	k_cat (min⁻¹)^b	K_{m MgATP} (mM)	k_cat/K_m (min⁻¹ mM⁻¹)	% wild-type k_cat/K_m	k_cat (min⁻¹)	K_m (mM)	k_cat/K_m (min⁻¹ mM⁻¹)	% wild-type k_cat/K_m
wild-type	2.58 ± 0.09	0.022 ± 0.003	115 ± 2	(100)	3.02 ± 0.01	0.018 ± 0.007	167 ± 5	(100)
K1119Q	0.082 ± 0.001	1.20 ± 0.7	0.068 ± 0.005	0.06	2.69 ± 0.06	0.040 ± 0.001	67.5 ± 0.3	40
T882A	14 ± 2	0.054 ± 0.002	260 ± 15	225	38 ± 7	0.09 ± 0.05	430 ± 10	260

Reaction conditions: 50 mM Bis-Tris, 25 mM Tricine, 25 mM glycine (pH 7.5), 25° C, 15 mM HCO₃⁻, 10 mM biotin, 7.5 mM MgCl₂, MgATP (0.09-3.0 mM), 0.25 mM acetyl-CoA.

Data fitted to eqn (1).