Skip to main content
. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Biochemistry. 2011 Oct 14;50(45):9694–9707. doi: 10.1021/bi201276r

Table 8.

Inhibition of pyruvate carboxylation and MgADP phosphorylation by phosphonoacetate with respect to MgATP or carbamoyl phosphate

Pyruvate carboxylationa ADP phosphorylationb
kcat (min−1) 620 ± 25 kcat (min−1) 3.25 ± 0.02
Km (mM) 0.36 ± 0.03 Km (mM) 5.20 ± 0.06
Kis (mM) 2.6 ± 0.3 Kis (mM) 0.027 ± 0.003
Kii (mM) 18 ± 7 Kii (mM) 0.021 ± 0.001
Kid (mM) 1.9 ± 0.3
a

Data were fitted eqn (9) with MgATP as the variable substrate.

b

Data were fitted to eqn (10) describing hyperbolic noncompetitive inhibition with respect to carbamoyl phosphate.