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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Metab Eng. 2011 Sep 1;13(6):656–665. doi: 10.1016/j.ymben.2011.08.002

Figure 2. Overview of INST-MFA procedure.

Figure 2

Following introduction of 13C-labeled bicarbonate, a series of metabolite samples were obtained by rapid quenching and extraction of cyanobacterial cells. Dynamic changes in isotope labeling patterns were assessed using GC-MS and LC-MS/MS, followed by computational analysis of these trajectories to estimate metabolic pathway fluxes.

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