Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Metab Eng. 2011 Sep 1;13(6):656–665. doi: 10.1016/j.ymben.2011.08.002

TABLE I. Isotope labeling measurements used for metabolic flux determination.

(a) LC-MS/MS ions and (b) GC-MS ions used to assess metabolite labeling. Standard errors of measurement (SEM) were determined based on the lack of agreement between measured and theoretically computed mass isotopomer distributions obtained from unlabeled cell extracts.

(a) LC-MS/MS ions

Metabolite Mass Carbons Composition SEM Reference
3PGA 185 1-2-3 C3H6O7P 0.5 (Luo et al., 2007)
DHAP 169 1-2-3 C3H6O6P 1.3 Ibid.
F6P 259 1-2-3-4-5-6 C6H12O9P 1.7 Ibid.
G6P 259 1-2-3-4-5-6 C6H12O9P 1.4 Ibid.
GAP 169 1-2-3 C3H6O6P 1.3 Ibid.
PEP 167 1-2-3 C3H4O6P 0.5 Ibid.
R5P 229 1-2-3-4-5 C5H10O8P 1.5 Ibid.
RUBP 309 1-2-3-4-5 C5H11O11P2 0.7 Ibid.
S7P 289 1-2-3-4-5-6-7 C7H14O10P 2.8 Ibid.
RU5P 229 1-2-3-4-5 C5H10O8P 1.3 Ibid.

(b) GC-MS ions

Metabolite Mass Carbons Composition SEM Reference

3PGA 357 2-3 C11H30O5PSi3 1.0 (Kitson et al., 1996)
3PGA 459 1-2-3 C14H36O7PSi4 1.0 Ibid.
CIT 273 1-2-3-4-5 C11H21O4Si2 1.0 (Tisza, 1994)
CIT 363 1-2-3-4-5 C14H31O5Si3 1.0 Ibid.
CIT 375 1-2-3-4-5-6 C14H27O6Si3 1.6 (Huege et al., 2007)
CIT 465 1-2-3-4-5-6 C17H37O7Si4 1.1 Ibid.
FUM 245 1-2-3-4 C9H17O4Si2 1.0 Ibid.
GA 292 1-2 C11H28O3Si3 1.0 (Petersson, 1970)
GA 307 1-2-3 C11H27O4Si3 1.3 (Huege et al., 2007)
MAL 233 2-3-4 C9H21O3Si2 1.0 (Petersson, 1972)
SUC 247 1-2-3-4 C9H19O4Si2 1.0 (Huege et al., 2007)
HHS Vulnerability Disclosure