Table 3.
Analysis of regenerated shoots from explants transformed with MYB10 or GUS gene construct and selected on kanamycin (50 mg/l) or not
| Species | Gene | Kanamycin added | No. of explants inoculated | No. of shoots tested | No. of shoots with visible phenotype (PCR +) | No. of shoots without visible phenotype (PCR +) | % transformation efficiency for visible phenotype* |
|---|---|---|---|---|---|---|---|
| Apple | MYB10 | No | 150 | 29 | 14a (14) | 15 (6) | 9 |
| Apple | MYB10 | Yes | 95 | 25 | 19a (19) | 6 (3) | 20 |
| Apple | GUS | No | 150 | 53 | 3b | 50 | 2 |
| Apple | GUS | Yes | 95 | 6 | 5b | 1 | 5 |
| Strawberry | MYB10 | No | 50 | 23 | 3 (3) | 20 (4) | 6 |
| Strawberry | MYB10 | Yes | 50 | 31 | 5 (5) | 26 (7) | 10 |
| Strawberry | GUS | No | 50 | 18 | 0 | 18 | 0 |
| Strawberry | GUS | Yes | 50 | 16 | 1 | 15 | 2 |
| Potato | MYB10 | No | 150 | 27 | 10** (10) | 17 (4) | 7 |
| Potato | MYB10 | Yes | 75 | 28 | 13** (13) | 15 (6) | 17 |
| Potato | GUS | No | 150 | 30 | 13 | 17 | 9 |
| Potato | GUS | Yes | 75 | 33 | 18 | 15 | 24 |
These shoots were checked for visible phenotype red anthocyanin accumulation or blue GUS staining, and analysed by PCR for the presence of the MYB10 gene construct (number in brackets)
* Percentage of explants producing a shoot with a visible phenotype
** After incubation in acid methanol
Values with a differ significantly from b with P value < 0.05
nd not determined