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. 2011 Aug 26;66A(12):1286–1299. doi: 10.1093/gerona/glr125

Figure 10.

Figure 10.

Levels of ASK1/Trx1 complex, JNK phosphorylation, and inflammatory gene expression in Tg(TRX1)+/0 and wild-type (WT) mice. Levels of ASK1/Trx1 complex and phosphorylated JNK were measured by Western blot analysis in cytosolic liver fractions from young mice (4–6 months old). Western blots for ASK1/Trx1 complex and phosphorylated JNK are shown with their respective graphs. The expression of inflammatory genes was measured by the levels of interleukin (IL)-1β, CCL5, and tumor necrosis factor (TNF) α using quantitative real-time polymerase chain reaction. Levels of ASK1/Trx1 complex were significantly higher in Tg(TRX1)+/0 compared with WT mice (Graph A: left). Levels of phosphorylated JNK were significantly lower in Tg(TRX1)+/0 compared with WT mice (Graph B: middle). Levels of IL-1β messenger RNA (mRNA) were significantly lower in the livers from young (4–6 months old) Tg(TRX1)+/0 mice compared with WT mice (Graph C: right). Levels of CCL5 (chemokine) and TNF-α mRNA were similar in the livers from young (4–6 months old) Tg(TRX1)+/0 and WT mice. The data are the mean of three to five animals ± SEM. The asterisk denotes those values that are significantly different from WT mice at the p ≤ .05 level.