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. Author manuscript; available in PMC: 2012 May 16.
Published in final edited form as: Chem Res Toxicol. 2011 Apr 12;24(5):726–736. doi: 10.1021/tx2000152

Figure 1.

Figure 1

Dose-dependent inhibition of pre-reduced TrxR by illudin S and AFs. TrxR (80 nM) was first incubated with NADPH (100 μM) at 25 °C for 10 min followed by the addition of test compounds and further incubated for 2 h at 25 °C (A, Illudin S, 62.5, 125, 250, 500, 1000, 2500 μM; B, AF, 2.5, 5, 12.5, 25, 50 μM; C, HMAF, 0.125, 0.25, 0.5, 1, 1.5, 2.5 μM). The residual activity was measured with DTNB assay described in the experimental details.