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. Author manuscript; available in PMC: 2012 May 16.
Published in final edited form as: Chem Res Toxicol. 2011 Apr 12;24(5):726–736. doi: 10.1021/tx2000152

Figure 4.

Figure 4

Gel-filtration analysis of AF and HMAF-inactivated TrxR. TrxR (80 nM) was first incubated with NADPH (100 μM) at 25 °C for 10 min followed by the addition of test compounds and further incubation for 2 h at 25 °C (A, AF, 3.3, 6.7, 16.7, 33.3, 66.7 μM; B, HMAF, 0.13, 0.27, 0.53, 1.33, 2.67 μM). Unbound compound was removed by Micro Bio-Spin™ P-6 pre-packed size exclusion columns. The residual activity was measured with DTNB assay described in the experimental details.