Figure 7.

SDS-PAGE gel blots probed with antibodies against soybean MS ACCase subunits. Soybean total seed (A) and pea leaf chloroplast stroma (B) extracts were probed. Lanes 1, Anti-BCCP; lanes 2, anti-BC; lanes 3, anti-β-CT (anti-pD-1x); lanes 4, anti-β-CT (anti-pD-3x); lanes 5, anti-α-CT (anti-pA1-3x); and lanes 6, anti-α-CT (anti-pA1-4x). (See Table II for overview of expression constructs.) Membranes were treated with both immune (lanes a) and preimmune (lanes b) sera. Whole membranes were cut into strips and each was incubated with a different antibody. Primary antibodies were used at 1:10,000 dilutions.