Figure 8.

Analysis of column fractions from total soybean seed extracts after chromatography through antibody-affinity columns. Lanes 1, Protein-gel blot probed with streptavidin-alkaline phosphatase conjugate; lanes 2, anti-BCCP probe; lanes 3, anti-BC probe; lanes 4, anti-α-CT probe; lanes 5, anti-β-CT probe. Gly eluates from each column (indicated above the lanes) were separated on 9% SDS-PAGE and transferred to PVDF membranes. Each membrane was subsequently cut into strips and probed with each ACCase antibody.