Table II.

Summary of the E. coli expression plasmids encoding ACCase proteins

Name	Vector	Insert Size	Subunit	Expected Protein Size	Size of Expressed Protein (SDS-PAGE)
		bp		kD
pA1-1x	pET-16b	2487	α-CT-1 precursor, 709 aaa	80.9	No expression
pA1-2x	pGEMEX-1	2487	α-CT-1 precursor, 709 aa	78.5	No expression
pA2-1x	pET-16b	2247	α-CT-2 precursor, 690 aa	79.3	No expression
pA2-2x	pGEMEX-1	2247	α-CT-2 precursor, 690 aa	76.9	No expression
pB-1x	pET-16b	1068	BCCP precursor, 262 aa	30	∼40
pB-2x	pGEMEX-1	1068	BCCP precursor, 262 aa	27.6	∼38
pC-1x	pET-16b	850 (PCR fragment)	BC (fragment), 273 aa	32.4	∼36
pC-2x	pGEMEX-1	1940	BC-2 precursor, 539 aa	58.9	53
pD-1x	pET-16b	1350	β-CT, 432 aa	51.3	∼50
pA1-3x	pET-16b	680	α-CT-1 fragment, aa 454-681, total 228 aa	27.4	∼30
pA1-4x	pET-16b	969	α-CT1, fragment, aa (158–453) + (682–709), total 324 aa	38.3	∼40
pD-3x	pET-16b	660	β-CT fragment, aa 1-223	27.2	∼36

^aaa, Amino acid(s).