Table 3.
Primer sequence and reaction conditions for PCR amplification.
Gene | Primer | Primer sequence 5′-3′ | Reaction conditions |
---|---|---|---|
psbA-trnH | Forward | GTTATGCATGAACGTAATGCTC | 94 °C 1 min, 94 °C 30 s, 53 °C 40 s, 72 °C 30 s, 40 cycles, 72 °C 5 min |
Reverse | CGCGCATGGTGGATTCACAAATC | ||
rpoB | Forward | ATGCAACGTCAAGCAGTTCC | 94 °C 1 min, 94 °C 30 s, 53 °C 40 s, 72 °C 30 s, 40 cycles, 72 °C 5 min |
Reverse | GATCCCAGCATCACAATTCC |