Figure 3.

Maximum-likelihood phylogenetic tree of NAD-dependent DNA ligases with metagenomic reads from the marine vent virome. The “large read cluster” denotes the branch in which the majority of metagenomic reads matching ligases grouped on the tree. Numbers indicate the bootstrap values of internal nodes (where n = 100). Metagenomic reads are colored red. All ligase protein sequences were obtained from NCBI (accession numbers listed). Trees were constructed in RAxML by incorporating metagenomic sequences into a constraint tree of references sequences based on the phylogeny of Yutin and Koonin (2009). Trees imaged with TreeViewX.