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. Author manuscript; available in PMC: 2011 Nov 9.
Published in final edited form as: J Immunol. 2011 Jan 31;186(5):3058–3065. doi: 10.4049/jimmunol.1001791

Figure 4. Allele-specific protein-DNA interactions.

Figure 4

A, EMSA using radiolabelled probes corresponding to the two alleles of rs522807 show allele-specific differences in binding when incubated with nuclear extracts for U937 cells, either unstimulated (○) or after induction with LPS for 6 h (●). B, The specificity of these interactions was confirmed on competition with 10- or 100-fold molar excess of unlabelled probe corresponding to each of the two alleles, a CRE binding site or an unrelated sequence (corresponding to a YY1 binding site).