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. Author manuscript; available in PMC: 2012 Dec 5.
Published in final edited form as: Virology. 2011 Oct 4;421(1):12–18. doi: 10.1016/j.virol.2011.09.002

Figure 4. Foxp3 interacts with HDAC1.

Figure 4

(A) 293T cells cotransfected with flag-HDAC1 and myc-tagged Foxp3 were Immunoprecipitated with anti-Flag antibody. (B) Jurkat cells transduced with myc-Foxp3 were Immunoprecipitated with anti-myc antibody and probed for endogenous HDAC1. (C) Forkhead mutation ablates Foxp3:HDAC1 interaction. 293T cells were transfected with myc-Foxp3 mutants with deletion or point mutations and Flag-HDAC1. HDAC1 was immunoprecipitated with anti-Flag antibody and Foxp3 was visualized by western blot. Total 10% of input cell lysate was included as controls. ZF-LZ-FKH, Myc-tagged FoxP3 with the N-terminal 177 amino acids deleted; LZ-FKH, Myc-tagged FoxP3 with the N-terminal 230 amino acids deleted; FKH, Myc-tagged FoxP3 with the N-terminal 311 amino acids deleted; WRR and WWRR are full-length FoxP3 mutants with point mutations in the FKH domain (5).