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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Biochemistry. 2011 Oct 13;50(45):9724–9737. doi: 10.1021/bi2012788

Figure 2.

Figure 2

Reciprocal plots demonstrating the linear, uncompetitive inhibition with respect to MgATP by free biotin for the bicarbonate-dependent ATPase reaction catalyzed by the R353M/K1119Q RePC double mutant. Solid lines indicate the least-square fits to eqn (3) at varying concentrations of MgATP (0.09–3.0 mM) and fixed concentrations of biotin (0 mM, black; 1 mM, red; 3 mM, blue; 5 mM, green; 7 mM, pink; 10 mM, cyan).