Table 5.

Specific activities of the biotin carboxylase domain mutants for the oxamate induced decarboxylation of oxaloacetate^a.

	kcat (min−1)b	% wild- type rate
wild-type	9.03 ± 0.04	(100)
E218A	0.42 ± 0.01	4
E218Q	0.59 ± 0.01	6
K245Q	2.01 ± 0.01	22
E305A	3.28 ± 0.07	36
E305Q	1.27 ± 0.02	14
E305D	7.45 ± 0.05	82
R301Q	6.0 ± 0.1	66
R301K	5.67 ± 0.07	63
R353K	4.23 ± 0.09	47
R353M	5.8 ± 0.2	64
E305A/K1119Q	NAc	---
R353M/K1119Q	NA	---

^aReaction conditions: 50 mM Bis-Tris, 25 mM Tricine, 25 mM glycine (pH 7.5), 25° C, 1 mM oxamate, 0.95 mM oxaloacetate, 0.25 mM acetyl-CoA.

^bNumber of determinations = 3, error reported is standard deviations of 3 trials

^cNA = No activity detected.