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Separation of acid and alkaline α-galactosidases from C. melo fruit (10 DAA) on ion-exchange chromatography. The protein fraction of 5% to 50% (w/v) PEG-6000 was applied to a column of DEAE-Sepharose 4B and eluted with the indicated linear gradient of 0 to 0.45 m NaCl. Activity of each fraction was assayed with pNPG at pH 5.0 and pH 7.5. Zero activity points are not shown.
