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The calibration curve of Superdex 200H 10/30 from which the native molecular mass of the acid form and alkaline forms I and II α-galactosidases were determined. The molecular markers were: 1, β-amylase (200 kD); 2, alcohol dehydrogenase (150 kD); 3, BSA (66 kD); 4, carbonic anhydrase (29 kD); and 5, Cyt c (12.4 kD).
