Table I.

Purification scheme for the acidic form and alkaline forms I and II α-galactosidases from C. melo fruit

Purification Step	Enzyme Activity	Protein	Specific Activity	Recovery	Purification
	units	mg	units × 10 mg−1 protein	%	-fold
Acid form
Crude extract	13.0	1152	0.11	100	–
5% to 50% PEG fraction	11.4	481	0.24	88	2
DEAE-Sepharose	4.6	45	1.02	35	9
Sephadex-200	3.0	13	2.32	23	21
ConA	1.7	2.6	6.27	13	55
Alkaline form I
Crude extract	27.7	1152	0.24	100	–
5% to 50% PEG fraction	20.7	481	0.43	75	2
DEAE-Sepharose	9.5	35	2.73	34	11
Mono-Q	8.0	15	5.37	29	22
Bio-gel HTP	5.1	3.7	13.82	19	57
Alkaline form II
Crude extract	18.4	1152	0.16	100	–
5% to 50% PEG fraction	16.4	481	0.34	89	2
DEAE-Sepharose	10.9	58	1.89	59	12
Mono-Q	8.8	17	5.21	48	33
Phenyl Sepharose 6 fast flow	5.8	2.9	19.73	31	123

All specific activities were assayed using raffinose (for acid and alkaline form I) or stachyose (for alkaline form II) as the substrate.