Table II.
Characterization of partially purified acidic and alkaline forms I and II α-galactosidases from C. melo fruit
| α-Galactosidase | Substrate | Km | Vmax | Vmax/Km |
|---|---|---|---|---|
| mm | μmol mg−1 protein min−1 | |||
| Acid form | Stachyose | 10.5 | 0.23 | 0.02 |
| Raffinose | 4.2 | 0.71 | 0.17 | |
| Melibiose | 0.7 | 0.19 | 0.27 | |
| pNPG | 0.3 | 1.5 | 5.00 | |
| Alkaline form I | Stachyose | 4.0 | 0.24 | 0.06 |
| Raffinose | 1.5 | 0.56 | 0.37 | |
| Melibiose | 20 | 0.3 | 0.02 | |
| pNPG | 1.2 | 1.4 | 1.17 | |
| Alkaline form II | Stachyose | 3.6 | 2.2 | 0.61 |
| Raffinose | 26.3 | 0.26 | 0.01 | |
| Melibiose | 18.7 | 0.21 | 0.01 | |
| pNPG | 3.0 | 7.9 | 2.63 |