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. 2011 Aug 3;(54):2552. doi: 10.3791/2552
1. 2x Universal MM 13.75 μl
2. DEPC-Water 0.792 μl
3. MgCl2 (100 mM) 0.55 μl
4. dNTP (100 mM) 1.1 μl
5. x-plex forward primer mix (450 nM) 3.06 μl
6. Universal RP (100 μM) 1.375 μl
7. AmpliTaqGold polymerase (5 U/μl) 1.375 μl