1. 2x Universal MM | 13.75 μl |
2. DEPC-Water | 0.792 μl |
3. MgCl2 (100 mM) | 0.55 μl |
4. dNTP (100 mM) | 1.1 μl |
5. x-plex forward primer mix (450 nM) | 3.06 μl |
6. Universal RP (100 μM) | 1.375 μl |
7. AmpliTaqGold polymerase (5 U/μl) | 1.375 μl |
1. 2x Universal MM | 13.75 μl |
2. DEPC-Water | 0.792 μl |
3. MgCl2 (100 mM) | 0.55 μl |
4. dNTP (100 mM) | 1.1 μl |
5. x-plex forward primer mix (450 nM) | 3.06 μl |
6. Universal RP (100 μM) | 1.375 μl |
7. AmpliTaqGold polymerase (5 U/μl) | 1.375 μl |