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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mol Microbiol. 2011 Oct 10;82(3):634–647. doi: 10.1111/j.1365-2958.2011.07843.x

Fig. 1.

Fig. 1

In vivo Activity of AtxA-His and AtxA-FLAG. In an atxA-null strain (UT376), production of AtxA (pUTE658), AtxA-His (pUTE991), and AtxA-FLAG (pUTE992) was induced using 30 μM IPTG during growth in CACO3. The empty vector sample (EV) was derived from UT376 (pUTE657) which lacks atxA. Samples were obtained at the transition to stationary phase (4 h; OD600 1.2 to 1.7). (A) AtxA and RNA Polymerase β subunit in soluble cell lysates were detected via immunoblotting. The AtxA levels indicated were normalized relative to the corresponding RNA polymerase β level. (B) The β-galactosidase activity of B. anthracis mutants harboring the Plef-lacZ reporter and IPTG-inducible atxA alleles was determined as described previously (Miller, 1972).