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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mol Microbiol. 2011 Oct 10;82(3):634–647. doi: 10.1111/j.1365-2958.2011.07843.x

Fig. 2.

Fig. 2

Oligomeric states of AtxA. (A) Lysates from B. anthracis strains UT376 (pUTE657) and UT376 (pUTE658), containing an empty vector (EV) and IPTG-inducible atxA respectively, and affinity-purified AtxA-His were electrophoresed on BN polyacrylamide gels. AtxA protein was detected using Western blotting. M = molecular weight markers (ProSieve Unstained Protein Marker, VWR). (B) Affinity purified AtxA-His from B. anthracis UT376 (pUTE991) was subjected to SDS-PAGE and stained with Coomassie. M = molecular weight markers (SeeBlue Plus2 Pre-Stained Standard, Invitrogen).