Figure 1.

Structure of the ATHCK2A antisense chimeric gene used to transform Arabidopsis. The PCR product amplified from the coding region of the ATHCK2A cDNA was inserted into pCRII (lower diagram). The 5′ primer had an XbaI site for further cloning. The recombinant pCRII vector was cut with XbaI and XhoI, and the insert was ligated into a similarly cut binary vector, pKYLX71 (upper diagram). This process gave the antisense orientation of ATHCK2A under the control of the cauliflower mosaic virus (CaMV) 35S promoter. MCS, Multiple cloning sites; nos, nopaline synthase gene; Km^R, kanamycin-resistance gene; Tc^R, tetracyclin-resistance gene; RK2 ori, bacterial replication origin; T_R and T_L, right and left border of T-DNA, respectively.