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Southern-blot hybridization of genomic DNA from antisense ATHCK2A1 transformants. Genomic DNA was digested with HindIII, separated by electrophoresis, transferred to a nylon membrane, and hybridized with a 32P-labeled cauliflower mosaic virus 35S promoter region cut from pBI121 (Clontech). D, F, G, H, and M, Five different antisense transformants.
