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. 1999 Mar;119(3):989–1000. doi: 10.1104/pp.119.3.989

Figure 4.

Figure 4

Dot-blot analysis of ATHCKA1 binding to cDNA inserts of other protein kinases (A) and northern-blot analyses of cdc2 in wild type (WT) and the transformants (D, F, G, H, and M) (B). A, Either 100 ng (1×) or 200 ng (2×) of the 1.2-kb ATHCK2A1 cDNA insert (column 3, second lane) was loaded, and the amount of other DNAs (the number of molecules) was adjusted according to their size. As a negative control, the cDNA insert of CK2B1 and the plasmid 100 ng of pUC18 was loaded. Lane 1, CK2B1 insert (1.1 kb, SalI/XbaI cut); lane 2, pUC18; lane 3, CK2A1 insert (1.2 kb, SalI/XbaI cut); lane 4, cdc2a (1.4 kb, EcoRI cut); lane 5, clone 2F1T7P Arabidopsis protein kinase homolog (0.7 kb, SalI/XbaI cut); and lane 6, plant-specific protein-kinase clone CD-18 (0.63 kb, EcoRI cut). B, Northern-blot analyses of the expression of cdc2 kinase in the wild type and the transformants. Poly(A+) RNA was isolated from the total RNA extracted from 21-d-old light-grown plants and 0.5 μg was loaded in each lane. Riboprobes were synthesized as described in Methods. The probed blot was stripped off using a kit and reprobed.